Thursday, June 16, 2011

Taq polymerase

Taq polymerase – a DNA polymerase (an enzyme) used to amplify a specific DNA
template in the PCR technique.
VNTR (variable number of tandem repeats) – in DNA testing, a polymorphic
locus where alleles differ primarily in the number of times that a string of
nucleotides are tandemly repeated. Widely used throughout the 1990’s but largely
replaced by PCR-based STR testing today.

1 comment:

  1. Hello! I am glad to stop by your site and know more about taq. Keep it up! This is a good read. I will be looking forward to visit your page again and for your other posts as well. Thank you for sharing your thoughts about taq in your area.
    Use of the thermostable Taq polymerase eliminates the need for having to add new enzyme to the PCR reaction during the thermocycling process. A single closed tube in a relatively simple machine can be used to carry out the entire process. Thus, the use of Taq polymerase was the key idea that made PCR applicable to a large variety of molecular biology problems concerning DNA analysis.
    T4 RNA Ligase 2, Truncated requires a pre-adenylated 5' phosphate containing DNA or RNA to ligate to 3' hydroxyl of RNA. Does not require ATP.

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